Part:BBa_K2170052:Design

Secretory prokaryotic biotin binding receptor with single chain avidin

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 3735
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 889
  

Keywords:

Abbreviations:

Design Notes

Related BioBrick:

• Other versions:BBa_K2170050: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin
  
• Related BioBricks:BBa_K2170051: Secretory prokaryotic biotin presenting receptor with biotin acceptor peptide

Cloning details:

• Designed in RFC10

Quality control measures:

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: Secretory prokaryotic biotin binding receptor with enhanced monomeric avidin [Nucleotide 1030 to 2445]-->
• Tag: internal A3C5 Tag

Enzymatic activity:

• none

Cytotoxicity:

• none

Safety notes:
Known and anticipated sefety issues: none Known and anticipated security issues: none

Source

Source:

• Preexisting BioBrick BBa_I739001; BBa_B0015
• Parts Synthesized by IDT
• Professor Dr. Arne Skerra (Chair biological chemistry of TUM)

Organism:
Genesequence derived from Escherichia coli

• Designed for the following Chassis: procaryotic cells

References

Literature references:
1. [http://jcb.rupress.org/content/108/2/229.short Kozak, M. (1989). The scanning model for translation: an update. The Journal of cell biology, 108(2), 229-241.]
2. [http://journals.plos.org/plosone/article?id=10.1371/journal.pone.000439 Kredel, S., Oswald, F., Nienhaus, K., Deuschle, K., Röcker, C., Wolff, M., ... & Wiedenmann, J. (2009). mRuby, a bright monomeric red fluorescent protein for labeling of subcellular structures. PloS one, 4(2), e4391.]
3. Bajar, B. T., Wang, E. S., Lam, A. J., Kim, B. B., Jacobs, C. L., Howe, E. S., ... & Chu, J. (2016). Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting. Scientific reports, 6.
4. [http://www.nature.com/nprot/journal/v2/n6/abs/nprot.2007.209.html Schmidt, T. G., & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature protocols, 2(6), 1528-1535.]

Database references: